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Clinical Information: Cholesterol Test Service

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Cholesterol Test Service

MyHealthTest’s Cholesterol Test Service is a fingerprick blood test for lipids that includes both total cholesterol and triglycerides.

This test service was developed to improve the access and convenience of blood testing to help identify people with elevated lipid levels and encourage them to visit their healthcare provider for further testing where indicated.

The MyHealthTest Cholesterol Test (total cholesterol and triglycerides) was validated against Australian NATA and NPAAC guidelines to confirm that dried blood spot (DBS) results obtained are comparable to serum samples collected by traditional venous venepuncture and analysed at an external NATA accredited pathology laboratory.

Samples are stable for up to 20 days after collection and thermal stability testing confirmed that after holding dried blood spot samples at 46°C for two days there was no significant difference in the total cholesterol and triglyceride values obtained.

Individuals should be aware that due to ordinary daily activities, there can be significant variations in your cholesterol levels throughout the day.

The MyHealthTest DBS Triglyceride test is based on a method where glycerol is an intermediate product. The topical application at the sample collection site of moisturisers containing glycerol / glycerine may falsely elevate the Triglyceride test result. Individuals taking this test are advised in the instructions for use to delay using moisturisers until after sample collection.



Total Cholesterol

As cholesterol is found in the membranes of the red blood cells and is released in dried blood spot testing, a new clinical target concentration for the dried blood spot assay has been established. The MyHealthTest cholesterol test assay has clinically acceptable precision to be used as a screening tool to identify individuals who might be at greater risk of cardiovascular disease.



Figure 1. Cholesterol Bland Altman and Linear Regression analysis of serum vs DBS



Triglycerides

The MyHealthTest triglycerides test assay has clinically acceptable precision to be used as a screening tool to identify individuals who might be at greater risk of cardiovascular disease.



Figure 2. Triglycerides Bland Altman and Linear Regression analysis of serum vs DBS



Total CholesterolTriglycerides
Measurement Range:1 – 17 mmol/L0.2 – 11.4 mmol/L
Clinical target concentration<6.4 mmol/L <1.5 mmol/L
Repeatability: <5% <5.5%
Reproducibility:12.2% at 3.8 mmol/L, 10.2% at 7 mmol/L19.4% at 1 mmol/L, 8.4% at 3.7 mmol/L
Stability: ≤20 days≤20 days
Interferences:High or low haematocrit will affect the reported total cholesterol valuesGlycerol, from the topical application of moisturisers, can falsely elevate triglyceride results.

High or low haematocrit may affect the reported triglyceride values.